Add the mtt solution to the media, wait for the amount of time you want, transfer everything in a 50 ml tube, may be washing the culture vessel a couple of times with pbs, then spin down at the. I don't like the mtt assay. i had troubles too to get it running. additionally, i think it is a bad measure of cell viability, since it greatly depends on the metabolic status of the cells. try the sulphorhodamine b assay. that's what i use to do assays like yours.. The mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay was the first homogeneous cell viability assay developed for a 96-well format that was suitable for high throughput screening . the mtt tetrazolium assay technology has been widely adopted and remains popular in academic labs as evidenced by.
Shows in a block diagram form how the mtt assay can be substituted for the thymidine uptake assay. the mtt assay has fewer steps, uses fewer materials, and does not carry the added burden of radioactive waste disposal. application note 5. Cells that reduce mtt to formazan dyes, giving a purple color. it is commonly used to determine . cytotoxicity of potential medicinal agents and toxic materials, since these types of materials are expected tostimulate or inhibit cell viability and growth. here, a general protocol is described to carry out an mtt assay on different types of cells.. Hi, i'm performing cell cytotoxicity assay using xtt approach. i culture several lung cancer cell lines from atcc. i found several protocols in published reports how to perform mtt or xtt assay.
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